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Success suggest the effects with the benzodiazepine web site ligands zolpidem, zopiclone, Cl218872, L-655,708 and DMCM were being just about entirely removed in all mutated receptors as much as a 1 microM concentration. The effects of bretazenil, Ro15-1788 or abecarnil were removed in a few, but not all mutated receptors, suggesting which the gamma 2F77I mutation differentially influences the steps of these ligands in numerous receptor subtypes. Moreover, this point mutation also influences the efficacy of diazepam for enhancing GABA-induced chloride flux, suggesting which the amino acid residue gamma 2F77 may also be linked to the transduction from the outcome of benzodiazepines from binding to gating.

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